Enantiomeric differentiation of chiral diols remains analytically challenging, particularly for aliphatic diols and complex mixtures. We report a 19F NMR-based three-component derivatization system combining fluorinated arylboronic acids with chiral amino alcohols to enable sensitive, high-resolution chiral analysis. By optimizing fluorine position and amino alcohol structure, we achieved excellent enantiomeric resolution across a range of diols, with resolution values up to 98.88. In some cases, simplified 19F signal patterns were rationalized by computationally predicted thermodynamic preferences among diastereomeric adducts. The method accurately determined enantiomeric excess in both model mixtures and crude Sharpless asymmetric dihydroxylation products, closely matching chiral HPLC results. Notably, it enables simultaneous detection of multiple diols without signal overlap, outperforming conventional 1H NMR methods. This approach highlights the potential of boronic acid-amino alcohol systems as versatile platforms for high-throughput chiral analysis using 19F-{1H} NMR.

Room-temperature phosphorescence (RTP) and organic long persistent luminescence (OLPL) materials are the current research hotspots in the field of luminescence and have broad application prospects, but afterglow systems with stimulus response or dynamic changes are still rare. Herein, we report unexpected dual afterglow change induced by conformational freezing in difluoroboron beta-diketonate (BF2bdk) systems. The cyclized BF2bdk system (after conformational freezing) exhibits bright green RTP followed by weak OLPL, whereas noncyclized BF2bdk system (before conformational freezing) displays blue thermally activated delayed fluorescence (TADF)-type afterglow with subsequent stronger OLPL. In-depth investigations reveal that the inversion in afterglow intensity and mechanism change arise from the difference in Delta E ST as well as the ability to undergo charge separation and harvest of excitons. Besides, this dual afterglow changing behavior with brightness inversion over time demonstrates significant potential for advanced dynamic anticounterfeiting and multilevel information encryption applications.

Selenoproteins contain the selenocysteine (Sec, U), which is essential for redox regulation due to its reactive selenol group. The current set of 25 human selenoproteins was defined by the presence of SECIS elements in the 3' UTR coupled with in-frame UGA codons through a stop codon readthrough mechanism. However, the discovery of novel selenoproteins and SECIS elements remains limited by the constraints of SECIS prediction methods. In this study, we focus on SECISBP2, the core SECIS-binding protein, to analyze its binding RNAs using RNA immunoprecipitation sequencing (RIP-Seq) technology. We constructed the 3S-DB, a database of 1,333 SECISBP2-bound RNAs with potential SECIS functions for UGA recoding, including most known selenoprotein transcripts. Importantly, we validate that the 3 ' UTRs of PDF and ATP5MJ exhibit SECIS activity using luciferase assays and by fusing them to known selenoprotein RNAs. In summary, our results provide a valuable resource of mRNAs with potential for UGA recoding and previously unrecognized SECIS elements, with the potential to expand the known selenoproteome and advance our understanding of their roles in redox biology and beyond. Furthermore, this work offers new insights into non-canonical stop codon readthrough and the broader mechanisms governing translational regulation of the genetic code.

We report a method for catalyzing the oxidative dehydrogenation of tetrahydroquinoline and indoline derivatives under metal-free conditions. With 9-azabicyclo[3.3.1]nonane N-oxyl (ABNO) as the catalyst in the presence of potassium tert-butoxide (tBuOK), this approach facilitates the efficient oxidative dehydrogenation of partially saturated N-heterocyclic arylamines to their aromatic counterparts, offering an alternative approach for N-heterocycle synthesis.

We herein report the first enantioselective Rh-catalyzed reductive hydroformylation of alkenyl boronic esters. By employing either camouflage or direct reductive AHF strategy, enantiodivergent synthesis of chiral gamma-boryl alcohols has been developed for the first time in high yields and excellent enantioselectivities with alkene 1,2-diboronic esters or alkenyl boronic esters as the starting materials using rhodium/Ph-BPE as the catalyst. The method has enjoyed high chemo-selectivity, good functional group compatibility, and broad substrate scope. The chiral gamma-boryl alcohol or diol products are versatile building blocks applicable to the synthesis of a series of APIs including (S)-tolterodine, (S)-dapoxetine, and (R)-atomaxetine. DFT calculation has revealed the origin of enantiodivergence of Rh-catalyzed reductive AHF of alkenyl boronic esters with Ph-BPE as the chiral ligand. The transformation is promised to provide great synthetic potential in both academia and industry.

The efficient and selective synthesis of alkylamines is critically important for organic chemistry and drug discovery. Nevertheless, developing catalytic protocols that allow for switchable access to structurally diverse alkylamines from readily available precursors remains a significant challenge. While deoxygenation of amides/aldehydes offers efficient alkylamine synthesis, prior methods are largely confined to alpha-substituted products, limiting structural diversity. Herein, we report a strategy that overcomes this key limitation, enabling regiodivergent access to either alpha- or beta-allylated alkylamines with high selectivity. Starting from abundant amides and allylic halides, utilization of the in situ formed iminium or enamine intermediate dictates switchable regio-chemistry to provide a versatile platform for the synthesis of complex amines. Furthermore, a tandem Ni-Ir catalytic system achieves a one-step beta-allylated amine synthesis via three-component coupling of aldehydes, amines, and allylic halides, bypassing traditional alpha-selectivity. This methodology delivers switchable regiocontrol and broad scope from readily available starting materials, significantly expanding synthetic access to valuable alkylamine building blocks.